1. Product 설명
HyperSilMag-Amino 비드 are magnetic silica beads functionalized with primary amine groups. They are designed for the covalent coupling of 리간드s such as proteins, antibodies, or peptides containing carboxyl or aldehyde groups. The hydrophilic surface ensures low non-specific binding.
2. Specifications
- Bead Diameter: 1.0 µm
- 농도: 10 mg/mL
- 표면 그룹: 아민 (-NH2)
- 보관 버퍼: PBS, pH 7.4, 0.02% NaN3
- 보관 조건: 2-8°C. DO NOT FREEZE.
3. Required Materials (Not Included)
- 자성 분리기 (MagStand)
- 결합 버퍼: MES 버퍼 (pH 5.0) or PBS (pH 7.4) depending on linker
- Activation Reagents: EDC/NHS or Glutaraldehyde
- 세척 버퍼s: PBS, Tris-HCl
4. 결합 프로토콜 (Glutaraldehyde 방법)
- Wash: Transfer 100 µL of beads to a tube. 위치시키다 on magnet for 1 min, 상청액을 제거하십시오. PBS로 3회 세척하십시오. 보관 at 4°C.