HyperSilMag-Biotin (100mL)
High-affinity magnetic beads for biotinylated molecule isolation.
Bio-Separation Selective and Powerful
Selective and Powerful
Isolation.
HyperSilMag Biotin is a high-efficiency magnetic bead designed for selective and powerful isolation of streptavidin/avidin molecules.
With low non-specific binding properties and large surface area, it delivers exceptional performance in purification and separation of nucleic acids, proteins, cells, and more.
$11,500
| Ligand | Recombinant Streptavidin |
| Bead Size | < 100 nm |
| Binding Capacity | > 2500 pmol free Biotin / mg beads |
| Streptavidin Binding; > 30 ug / mg beads;'> > 30 ug Streptavidin / mg beads | |
| Concentration | 10 mg/mL |
| Blocking | BSA blocked (Low non-specific binding) |
Materials Required:
- HyperSilMag Biotin
- Streptavidin-conjugated target molecule (protein, nucleic acid, etc.)
- Binding Buffer (PBS with 0.05% Tween-20)
- Wash Buffer (PBS with 0.1% Tween-20)
- Elution Buffer (optional, for recovery)
- Magnetic separator
Procedure:
- Bead Preparation: Wash Biotin Beads 2x with Binding Buffer using magnetic separation.
- Binding: Resuspend beads in Binding Buffer. Add streptavidin-conjugated target (0.1-10 ug/mL). Incubate 15-30 minutes at room temperature with gentle rotation.
- Washing: Magnetically separate and wash 3x with Wash Buffer to remove unbound material.
- Application: Use beads directly for assays, or proceed to elution if target recovery is needed.
- Elution (Optional): Add Elution Buffer (e.g., biotin solution 5 mM). Incubate 10 minutes with mixing. Magnetically separate and collect eluate.
Binding Capacity: >3000 pmol biotin per mg beads
Affinity: Kd = 10^-15 M (one of the strongest non-covalent interactions)
| Cat. No. | Product Name | Size | Price |
|---|---|---|---|
| TS-BIO-010 | HyperSilMag-Biotin | 10mL | $1,500 |
| TS-BIO-025 | HyperSilMag-Biotin | 25mL | $3,400 |
| TS-BIO-050 | HyperSilMag-Biotin | 50mL | $6,300 |
| TS-BIO-100 | HyperSilMag-Biotin | 100mL | $11,500 |
| TS-BIO-500 | HyperSilMag-Biotin | 500mL | $50,000 |