HyperSilMag-Epoxy (10mL)
Epoxy-Activated Magnetic Silica Beads for Direct Coupling
Ready-to-Use One-Step Binding.
One-Step Binding.
No Activation Needed.
HyperSilMag-Epoxy beads feature a PEG shield for exceptional suspension stability. Reacts directly with amine, thiol, or hydroxyl groups for easy ligand coupling. Ideal for developing stable, low-background assays on automated platforms.
$1,500
| Surface Group | Epoxy (Glycidyl ether) |
| Bead Size | < 100 nm |
| Binding Chem | Covalent (Amine/Thiol/Hydroxyl) |
| Coupling Cond | pH 7-9 (Amine), pH 9-11 (Hydroxyl) |
| Concentration | 25 mg/mL |
| Storage | Dry powder or Isopropanol |
Materials Required:
- HyperSilMag-Epoxy beads
- Target molecule with amino, thiol, or hydroxyl groups
- Coupling buffer (0.1 M sodium phosphate pH 8.5-9.5)
- Wash buffer (PBS)
- Blocking solution (1 M Tris-HCl pH 8.0)
Procedure:
- Bead Washing: Wash epoxy beads 3횞 with coupling buffer.
- Coupling: Add target molecule (1-5 mg/mL) in coupling buffer. Incubate overnight at room temperature or 4-8 hours at 37째C with gentle rotation.
- Monitoring: Epoxy groups react with -NH2 (fastest), -SH (moderate), and -OH (slower). Adjust pH 8.5-9.5 for optimal amine coupling.
- Blocking: Add Tris blocking solution. Incubate 2-4 hours at room temperature to block remaining epoxy groups.
- Washing: Wash thoroughly 4횞 with PBS to remove unbound material and excess blocking agent.
- Storage: Store at 4째C in PBS with 0.02% sodium azide.
Binding Capacity: >20 mg protein per gram beads
| Cat. No. | Product Name | Size | Price |
|---|---|---|---|
| TS-EPX-010 | HyperSilMag-Epoxy | 10mL | $1,500 |
| TS-EPX-025 | HyperSilMag-Epoxy | 25mL | $3,400 |
| TS-EPX-050 | HyperSilMag-Epoxy | 50mL | $6,300 |
| TS-EPX-100 | HyperSilMag-Epoxy | 100mL | $11,500 |
| TS-EPX-500 | HyperSilMag-Epoxy | 500mL | $50,000 |