HyperSilMag-NTA (25mL)
NTA-Functionalized Magnetic Beads for His-tag Protein Purification
Surface
Activated
High Purity
High Purity
His-Tag Proteins
HyperSilMag-NTA magnetic beads are composed of a magnetic core coated with silica and a
PEG layer, functionalized with primary amines.
The PEG layer prevents sedimentation,
making these beads ideal for automated workflows.
| Ligand | Ni-NTA (Nitrilotriacetic acid) |
|---|---|
| Metal Ion | Nickel (Ni²⊎) |
| Bead Size | < 100 nm |
| Binding Capacity | > 40 mg His-tagged protein / mL beads |
| Concentration | 10 mg/mL |
| Regeneration | Strip with EDTA, Recharge with NiSO4 |
Materials Required:
- HyperSilMag-NTA beads (Charged with Ni²⊎ or Co²⊎)
- His-tag protein sample
- Binding Buffer (50 mM NaH2PO4, 300 mM NaCl, 10 mM imidazole, pH 8.0)
- Wash Buffer (50 mM NaH2PO4, 300 mM NaCl, 20 mM imidazole, pH 8.0)
- Elution Buffer (50 mM NaH2PO4, 300 mM NaCl, 250 mM imidazole, pH 8.0)
Procedure:
- Equilibration: Wash NTA beads 2x with Binding Buffer.
- Binding: Add sample to beads. Incubate 1 hour at 4°C with gentle rotation to allow His-tag binding to Ni-NTA.
- Magnetic Separation: Collect beads with magnet. Remove and save flow-through.
- Washing: Wash 3x with Wash Buffer (20 mM imidazole) to remove non-specifically bound proteins.
- Elution: Add Elution Buffer (250 mM imidazole). Incubate 5 min. Collect eluate containing purified His-tag protein.
- Regeneration: Strip nickel by washing with 0.5 M EDTA, then recharge with 50 mM NiSO4.
Yield: > 40 mg His-tag protein per gram of beads
Purity: Typically > 95% in a single step
| Cat. No. | Product Name | Size | Price |
|---|---|---|---|
| TS-NTA-010 | HyperSilMag-NTA | 10mL | $1,500 |
| TS-NTA-025 | HyperSilMag-NTA | 25mL | $3,400 |
| TS-NTA-050 | HyperSilMag-NTA | 50mL | $6,300 |
| TS-NTA-100 | HyperSilMag-NTA | 100mL | $11,500 |
| TS-NTA-500 | HyperSilMag-NTA | 500mL | $50,000 |