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HyperSilMag-Streptavidin (25mL)

High-affinity magnetic beads for biotinylated molecule isolation.

Overview Specifications Protocols Ordering Info
Bio-Separation

Selective and Powerful
Isolation.

HyperSilMag Streptavidin is a high-efficiency magnetic bead designed for selective and powerful isolation of biotinylated molecules.

With low non-specific binding properties and large surface area, it delivers exceptional performance in purification and separation of nucleic acids, proteins, cells, and more.

$3,400
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HyperSilMag Product

Product Specifications

Ligand Recombinant Streptavidin
Bead Size < 100 nm
Binding Capacity > 2500 pmol free Biotin / mg beads
Biotin-IgG Binding > 30 ug Biotin-IgG / mg beads
Concentration 10 mg/mL
Blocking BSA blocked (Low non-specific binding)

Biotin-Tagged Molecule Capture Protocol

Materials Required:

  • HyperSilMag Streptavidin
  • Biotinylated target molecule (protein, nucleic acid, etc.)
  • Binding Buffer (PBS with 0.05% Tween-20)
  • Wash Buffer (PBS with 0.1% Tween-20)
  • Elution Buffer (optional, for recovery)
  • Magnetic separator

Procedure:

  1. Bead Preparation: Wash streptavidin beads 2x with Binding Buffer using magnetic separation.
  2. Binding: Resuspend beads in Binding Buffer. Add biotinylated target (0.1-10 ug/mL). Incubate 15-30 minutes at room temperature with gentle rotation.
  3. Washing: Magnetically separate and wash 3x with Wash Buffer to remove unbound material.
  4. Application: Use beads directly for assays, or proceed to elution if target recovery is needed.
  5. Elution (Optional): Add Elution Buffer (e.g., biotin solution 5 mM). Incubate 10 minutes with mixing. Magnetically separate and collect eluate.

Binding Capacity: >3000 pmol biotin per mg beads

Affinity: Kd = 10^-15 M (one of the strongest non-covalent interactions)

Ordering Information

Cat. No. Product Name Size Price
TS-STR-010 HyperSilMag-Streptavidin 10mL $1,500
TS-STR-025 HyperSilMag-Streptavidin 25mL $3,400
TS-STR-050 HyperSilMag-Streptavidin 50mL $6,300
TS-STR-100 HyperSilMag-Streptavidin 100mL $11,500
TS-STR-500 HyperSilMag-Streptavidin 500mL $50,000